Review



anti gapdh reference control  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti gapdh reference control
    Anti Gapdh Reference Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/pm41845097-84-26-29
    Average 86 stars, based on 1 article reviews
    anti gapdh reference control - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Pan-cancer analysis reveals HDAC1 as a key regulator of immune infiltration and T cell exhaustion.
    Article Snippet: Protein resolution was achieved through SDS-PAGE, with subsequent electrotransfer to PVDF membrane surfaces (Vazyme, Cat# E801-01). .. Membrane blocking employed 5% non-fat milk solution (1-hour treatment), followed by primary antibody hybridization overnight at 4°C: anti-HDAC1 (Cell Signaling Technology, Cat# 34589T, 1:1000 dilution) and anti-GAPDH reference control (Cell Signaling Technology, Cat# 2118T, 1:5000). ..

    Blocking Assay:

    Article Title: Pan-cancer analysis reveals HDAC1 as a key regulator of immune infiltration and T cell exhaustion.
    Article Snippet: Protein resolution was achieved through SDS-PAGE, with subsequent electrotransfer to PVDF membrane surfaces (Vazyme, Cat# E801-01). .. Membrane blocking employed 5% non-fat milk solution (1-hour treatment), followed by primary antibody hybridization overnight at 4°C: anti-HDAC1 (Cell Signaling Technology, Cat# 34589T, 1:1000 dilution) and anti-GAPDH reference control (Cell Signaling Technology, Cat# 2118T, 1:5000). ..

    Hybridization:

    Article Title: Pan-cancer analysis reveals HDAC1 as a key regulator of immune infiltration and T cell exhaustion.
    Article Snippet: Protein resolution was achieved through SDS-PAGE, with subsequent electrotransfer to PVDF membrane surfaces (Vazyme, Cat# E801-01). .. Membrane blocking employed 5% non-fat milk solution (1-hour treatment), followed by primary antibody hybridization overnight at 4°C: anti-HDAC1 (Cell Signaling Technology, Cat# 34589T, 1:1000 dilution) and anti-GAPDH reference control (Cell Signaling Technology, Cat# 2118T, 1:5000). ..

    Control:

    Article Title: Pan-cancer analysis reveals HDAC1 as a key regulator of immune infiltration and T cell exhaustion.
    Article Snippet: Protein resolution was achieved through SDS-PAGE, with subsequent electrotransfer to PVDF membrane surfaces (Vazyme, Cat# E801-01). .. Membrane blocking employed 5% non-fat milk solution (1-hour treatment), followed by primary antibody hybridization overnight at 4°C: anti-HDAC1 (Cell Signaling Technology, Cat# 34589T, 1:1000 dilution) and anti-GAPDH reference control (Cell Signaling Technology, Cat# 2118T, 1:5000). ..



    Similar Products

    86
    Cell Signaling Technology Inc anti gapdh reference control
    Anti Gapdh Reference Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/pm41845097-84-26-29
    Average 86 stars, based on 1 article reviews
    anti gapdh reference control - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    Danaher Inc reference gapdh secondary ab
    Reference Gapdh Secondary Ab, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pmc10494775-29-106-110
    Average 99 stars, based on 1 article reviews
    reference gapdh secondary ab - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Danaher Inc internal reference gapdh antibodies
    Internal Reference Gapdh Antibodies, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pmc09850756-71-32-39
    Average 99 stars, based on 1 article reviews
    internal reference gapdh antibodies - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Danaher Inc internal reference anti gapdh
    Internal Reference Anti Gapdh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pm32716640-47-45-80
    Average 99 stars, based on 1 article reviews
    internal reference anti gapdh - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Danaher Inc reference materials gapdh
    Reference Materials Gapdh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pm34937832-54-20-23
    Average 99 stars, based on 1 article reviews
    reference materials gapdh - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Danaher Inc cytosolic reference gapdh
    Cytosolic Reference Gapdh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pm33819360-84-7-16
    Average 99 stars, based on 1 article reviews
    cytosolic reference gapdh - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Danaher Inc reference antibody gapdh
    Effects of Ononin on IL-1β-induced ECM degradation in Rat Chondrocytes. ( A ) Expression levels of Collagen II <t>and</t> <t>MMP-13</t> were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. ( B , C ) The expression level of target proteins was expressed by the ratio of grey value of target band to <t>GAPDH</t> band by Image-J software. ( D ) Immunofluorescence staining of Collagen II protein (green) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( E ) Immunofluorescence staining of MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( F , G ) The summary data of fluorescence intensity of Collagen II and MMP-13 in situ. All experiments were performed in 3 times and data were expressed by mean ± SD. * P < 0.05 compared with control group; # P < 0.05 compared with IL-1β group; ** P < 0.01 compared with control group; ## P < 0.01 compared with IL-1β group
    Reference Antibody Gapdh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pmc08793192-34-12-18
    Average 99 stars, based on 1 article reviews
    reference antibody gapdh - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Danaher Inc internal reference gene gapdh
    Effects of Ononin on IL-1β-induced ECM degradation in Rat Chondrocytes. ( A ) Expression levels of Collagen II <t>and</t> <t>MMP-13</t> were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. ( B , C ) The expression level of target proteins was expressed by the ratio of grey value of target band to <t>GAPDH</t> band by Image-J software. ( D ) Immunofluorescence staining of Collagen II protein (green) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( E ) Immunofluorescence staining of MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( F , G ) The summary data of fluorescence intensity of Collagen II and MMP-13 in situ. All experiments were performed in 3 times and data were expressed by mean ± SD. * P < 0.05 compared with control group; # P < 0.05 compared with IL-1β group; ** P < 0.01 compared with control group; ## P < 0.01 compared with IL-1β group
    Internal Reference Gene Gapdh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+reference+control/Anti-GAPDH+antibody+-+Loading+Control/pmc08661180-132-53-57
    Average 99 stars, based on 1 article reviews
    internal reference gene gapdh - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effects of Ononin on IL-1β-induced ECM degradation in Rat Chondrocytes. ( A ) Expression levels of Collagen II and MMP-13 were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. ( B , C ) The expression level of target proteins was expressed by the ratio of grey value of target band to GAPDH band by Image-J software. ( D ) Immunofluorescence staining of Collagen II protein (green) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( E ) Immunofluorescence staining of MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( F , G ) The summary data of fluorescence intensity of Collagen II and MMP-13 in situ. All experiments were performed in 3 times and data were expressed by mean ± SD. * P < 0.05 compared with control group; # P < 0.05 compared with IL-1β group; ** P < 0.01 compared with control group; ## P < 0.01 compared with IL-1β group

    Journal: BMC Complementary Medicine and Therapies

    Article Title: Ononin ameliorates inflammation and cartilage degradation in rat chondrocytes with IL-1β-induced osteoarthritis by downregulating the MAPK and NF-κB pathways

    doi: 10.1186/s12906-022-03504-5

    Figure Lengend Snippet: Effects of Ononin on IL-1β-induced ECM degradation in Rat Chondrocytes. ( A ) Expression levels of Collagen II and MMP-13 were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. ( B , C ) The expression level of target proteins was expressed by the ratio of grey value of target band to GAPDH band by Image-J software. ( D ) Immunofluorescence staining of Collagen II protein (green) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( E ) Immunofluorescence staining of MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). ( F , G ) The summary data of fluorescence intensity of Collagen II and MMP-13 in situ. All experiments were performed in 3 times and data were expressed by mean ± SD. * P < 0.05 compared with control group; # P < 0.05 compared with IL-1β group; ** P < 0.01 compared with control group; ## P < 0.01 compared with IL-1β group

    Article Snippet: Rat cartilage-specific antibodies, such as MMP-13 and Collagen II, and the internal reference antibody GAPDH were purchased from Abcam (Cambridge, UK).

    Techniques: Expressing, Western Blot, Software, Immunofluorescence, Staining, Fluorescence, Microscopy, In Situ, Control